Journal: Materials Today Bio

Article Title: Porcine pericardial decellularized matrix bilayer patch containing adipose stem cell-derived exosomes for the treatment of diabetic wounds

doi: 10.1016/j.mtbio.2024.101398

Figure Lengend Snippet: Fig. 10. The exosome-loaded bilayer decellularized pericardial patches promote the healing of diabetic refractory wounds by enhancing the activation of M2 macrophages in the wound and activating the cAMP signaling pathway. (A) On day 7, dual immunofluorescence staining for F4/80 (M0 marker), iNOS (M1 marker), and CD206 (M2 marker) was performed to assess the numbers of activated M1 and M2 macrophages in the wound and surrounding skin tissue, scale bar = 50 μm. (B) On day 7, RT-qPCR was used to detect the expression levels of M1 (cd86, il-6) and M2 (cd163, arg-1) macrophage-related genes in the wound tissue of each group. (C) On day 7, Western Blotting experiments were conducted to measure the expression levels of key proteins in the cAMP signaling pathway (Atp1a2, Calm4, and Cngb1) in the wound tissue of each group, with quantitative analysis of protein density, n = 3 (D). *, p < 0.05, **, p < 0.01, and ***, p < 0.001, compared with the PBS group.

Article Snippet: The primary antibodies used were as follows: Ki67 (13180-T48, Sino Biological Inc, China), 1:500; VEGF (ab32152, Abcam, USA), 1:2000; Atp1a2 (ab166888, Abcam, USA), 1:2000; Calm4 (CSB-PA004454GA01HU, Cusabio), 1:1000; Gngb1 (PA5-101543, Invitrogen), 1:1000; GAPDH (#2118S, CST), 1:1000.

Techniques: Activation Assay, Immunofluorescence, Staining, Marker, Quantitative RT-PCR, Expressing, Western Blot